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The b GenScript SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Kit /b can detect circulating neutralizing antibodies against SARS-CoV-2 that block the interaction between the receptor binding domain of the viral spike glycoprotein (RBD) with the ACE2 cell surface receptor The assay detects any antibodies in serum and plasma that neutralize the RBD-ACE2 interaction The test is both species and isotype independent br/ br/ The b SARS-CoV-2 sVNT Kit /b is a blocking ELISA detection tool which mimics the virus neutralization process The kit contains two key components the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD) and the human ACE2 receptor protein (hACE2) The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2 RBD br/ First the samples and controls are pre-incubated with the HRP-RBD to allow the binding of the circulating neutralization antibodies to HRP-R
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SARS-CoV-2 the virus responsible for COVID-19 is an enveloped positive-sense single-stranded RNA virus It contains RNA and structural proteins necessary for host cell invasion Once inside the cell the virus uses its RNA to encode structural and nonstructural proteins involved in virus assembly replication and host interaction The virus s structural proteins include the envelope (E) spike (S) membrane (M) and nucleocapsid (N) proteins The spike protein responsible for the virus s crown-like appearance mediates attachment and entry into host cells The S protein is a key target for vaccines antibody therapies and diagnostic tests
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This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 Nucleocapsid (Catalog#40588-V08B; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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The product is specific for N501 site of wild-type RBD For specific mutant protein the product recognizes wild-type RBD RBD single mutant (E484K) and RBD single mutant (K417N) but not recognizes RBD single mutant (N501Y) and RBD triple mutant (K417N E484K and N501Y) as seen in Figure 2
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